M1219 is a dual near-infrared activated fluorescent probe for GSH and ATP, capable of independently monitoring the dynamic changes of intracellular GSH and ATP in real-time without spectral crosstalk (GSH: Ex=640 nm, Em=740~800 nm; ATP: Ex=594 nm/610 nm, Em=650~700 nm). It visualizes the metabolic regulation mechanism under SLC7A11/GLUT1 single/double target inhibition in TNBC and precisely assesses its in vivo efficacy, as well as accurately locates the tumor infiltration boundary in TNBC. M1219 is applicable for research in triple-negative breast cancer.