LCP is a fluorescent probe suitable for subcellular localization. It responds to changes in cell microenvironment polarity through fluorescence resonance energy transfer, emitting blue fluorescence in low-polarity environments and red fluorescence in high-polarity environments. LCP enables dual-color visualization of lysosomal and cytoplasmic membrane dynamics during drug-induced apoptosis and monitors cell viability through localization and emission color changes. LCP is applicable for cancer research.