Hancockiamide B, an N-cinnamoylated piperazine fungal metabolite identified in A. hancockii, exhibits antibacterial and anticancer properties. It is effective against B. subtilis with a minimum inhibitory concentration (MIC) of 50 µg/ml. Furthermore, Hancockiamide B is cytotoxic to NS-1 murine myeloma cells at an MIC of 25 µg/ml, yet it displays no toxicity to neonatal foreskin fibroblasts at a concentration of 100 µg/ml.