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Using specific antisera raised against synthetic peptides, we find that three distinct GTP-binding protein alpha subunits remain bound to the plasma membrane even after activation with nonhydrolyzable GTP analog. Trypsin cleaves each alpha subunit at a site near the amino-terminus and quantitatively releases the large fragment from the membrane. Previous results indicated that alpha subunits are essentially cytoplasmic proteins tethered to the inner surface of the membrane via an amino-terminal stalk.
Using specific antisera raised against synthetic peptides, we find that three distinct GTP-binding protein alpha subunits remain bound to the plasma membrane even after activation with nonhydrolyzable GTP analog. Trypsin cleaves each alpha subunit at a site near the amino-terminus and quantitatively releases the large fragment from the membrane. Previous results indicated that alpha subunits are essentially cytoplasmic proteins tethered to the inner surface of the membrane via an amino-terminal stalk.

| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 1 mg | ¥ 348 | 待询 | |
| 5 mg | ¥ 978 | 待询 | |
| 10 mg | ¥ 1,608 | 待询 | |
| 25 mg | ¥ 2,238 | 待询 |
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| 产品描述 | Using specific antisera raised against synthetic peptides, we find that three distinct GTP-binding protein alpha subunits remain bound to the plasma membrane even after activation with nonhydrolyzable GTP analog. Trypsin cleaves each alpha subunit at a site near the amino-terminus and quantitatively releases the large fragment from the membrane. Previous results indicated that alpha subunits are essentially cytoplasmic proteins tethered to the inner surface of the membrane via an amino-terminal stalk. |
| 分子量 | 1623.89 |
| 分子式 | C66H118N20O23S2 |
| Smiles | N[C@@]([H])(C[S])C(=O)NCC(=O)N[C@](C)([H])C(=O)NCC(=O)N[C@@]([H])(CCC(O)=O)C(=O)N[C@@]([H])(CO)C(=O)NCC(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CO)C(=O)N[C@]([H])(C(=O)N[C@]([H])(C(=O)N[C@]([H])(C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCC(N)=O)C(=O)N[C@@]([H])(CC[S]C)C(=O)N[C@@]([H])(CCCCN)C(=O)O)C(C)(C)[H])[C@@](C)([H])CC)[C@](C)(O)[H] |
| 密度 | no data available |
| Sequence | H2N-Cys-Gly-Ala-Gly-Glu-Ser-Gly-Lys-Ser-Thr-Ile-Val-Lys-Gln-Met-Lys-OH |
| Sequence Short | CGAGESGKSTIVKQMK |
| 存储 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. 实际储存温度请以COA为准 |
| 溶解度信息 | DMSO: ≥162.3 mg/mL, Sonication is recommended. |
对于不同动物的给药剂量换算,您也可以参考 更多