Exo-β-1,4-xylosidase, Bacteroides ovatus (EC.3.2.1.37), is a specialized exonuclease that targets the non-reducing end β-1,4 glycosidic bonds of xylan and xylooligosaccharides. This enzyme is Ca2+-dependent and facilitates the hydrolysis of β-1,4 glycosidic bonds through reversible metal ion binding, thereby degrading xylan to produce xylose. It is applicable in research areas such as lignocellulose bioconversion, bioethanol production, and optimization of xylan saccharification processes.