DPPE-PEG5000 is a PEG lipid functional terminal group utilized for synthesizing liposomes (LPs) and designing conjugated polymer nanoparticles. Liposome nanoparticles (LNPs) with biotin modification and carboxyl ends can further couple with other biomolecules. These functionalized nanoparticles target specific cellular proteins for labeling. Using streptavidin as a linker, the biotinylated PEG lipid-conjugated polymer nanoparticles can bind to biotinylated antibodies on cell surface receptors, enabling applications in fluorescence-based imaging and sensing.