DPPE-PEG3000 is a PEG lipid functional end group used for synthesizing liposomes (LPs) and designing conjugated polymer nanoparticles. Liposome nanoparticles (LNPs) modified with biotin and having a carboxylate end can further couple with other biomolecules. The functionalized nanoparticles are applicable for targeted labeling of specific cell proteins. Using streptavidin as a linker, biotinylated PEG lipid-conjugated polymer nanoparticles can interact with biotinylated antibodies on cell surface receptors, facilitating fluorescence-based imaging and sensing applications.