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Difluorocarboxyfluorescein NHS Ester, 6-isomer是荧光素的氟化类似物,可以与胺反应。它提供了更高的光稳定性和更低的pKa值(约4.7,相较于荧光素的6.4),在生理pH范围内其荧光对pH变化几乎不敏感。虽然二氟羧基荧光素NHS酯的混合异构体是标记蛋白质、肽和核苷酸的首选荧光染料,但在HPLC纯化中,5(6)异构体标记的肽和核苷酸信号可能会显著增宽,这会导致纯化的麻烦。用单一异构体标记的肽和核苷酸在HPLC纯化中通常具有更好的分辨率,这在结合过程中常常是必要的。
Difluorocarboxyfluorescein NHS Ester, 6-isomer是荧光素的氟化类似物,可以与胺反应。它提供了更高的光稳定性和更低的pKa值(约4.7,相较于荧光素的6.4),在生理pH范围内其荧光对pH变化几乎不敏感。虽然二氟羧基荧光素NHS酯的混合异构体是标记蛋白质、肽和核苷酸的首选荧光染料,但在HPLC纯化中,5(6)异构体标记的肽和核苷酸信号可能会显著增宽,这会导致纯化的麻烦。用单一异构体标记的肽和核苷酸在HPLC纯化中通常具有更好的分辨率,这在结合过程中常常是必要的。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
10 mg | 待询 | 10-14周 | |
50 mg | 待询 | 10-14周 |
产品描述 | Difluorocarboxyfluorescein NHS Ester, 6-isomer, is a fluorescent analogue of fluorescein that reacts with amines. It addresses several limitations of fluorescein, offering enhanced photostability and a lower pKa (~4.7 compared to fluorescein's 6.4), resulting in fluorescence that remains largely pH-insensitive within physiological ranges. Although mixed isomers of difluorocarboxyfluorescein NHS ester are often used as routine fluorescent dyes for labeling proteins, peptides, and nucleotides, purifying peptides and nucleotides labeled with the 5(6) isomer can be challenging due to signal broadening during HPLC purification. Peptides and nucleotides labeled with a single isomer typically show improved resolution in HPLC purification, which is often necessary during conjugation processes. |
分子量 | 509.369 |
分子式 | C25H13F2NO9 |
CAS No. | 2702251-82-7 |
存储 | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
以上为“体内实验配液计算器”的使用方法举例,并不是具体某个化合物的推荐配制方式,请根据您的实验动物和给药方式选择适当的溶解方案。
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