Dichlorofluorescein is a fluorescent dye employed in various scientific experiments and applications. It serves as an absorption indicator for halogen determination, facilitates the study of intracellular molecular and ion movements, and enables detection and measurement of ROS production. Dichlorofluorescein is also utilized in researching the pathological mechanisms of oxidative stress-related diseases (e.g., Parkinson's disease, Alzheimer's disease).