活性氧(ROS)探针

BODIPY 581/591 C11 是一种荧光放射探针，用于指示模型膜系统和活细胞中的脂质过氧化和抗氧化功效。

H2DCFDA (DCFH-DA) 属于绿色荧光染料，是一种检测细胞内活性氧 (ROS) 的探针 (Ex/Em=488/525 nm)，具有细胞膜渗透性。

Dihydroethidium (PD-MY 003) 是一种过氧化物指示剂，在细胞质中具有蓝色荧光 (λex=518 nm，λem=616 nm)，被氧化后荧光消失，具有细胞渗透性。

ADHP (10-Acetyl-3,7-dihydroxyphenoxazine) 是荧光过氧化物酶底物，其λex=530 nm，λem=590 nm。

5-ROX, SE ([5-Carboxy-X-rhodamine, succinimidyl ester] *Single isomer*) 是一种标记试剂，用于制备电荷修饰的染料标记的双脱氧核苷酸三磷酸 (ddNTP) 以“直接加载”DNA 测序。

1,3-Diphenylisobenzofuran (DPBF) 是一种荧光探针，对单线态氧(1O2) 的具有高特异性，可形成内过氧化物并分解成 1,2-dibenzoylbenzene，可用于活性氧生成的检测。

2',7'-Dichlorofluorescein diacetate (Diacetyldichlorofluorescein) 是一种可渗透细胞的荧光探针，可用于检测一氧化氮和活性氧以及确定总体氧化应激的程度。

Lucigenin (NSC-151912) 是化学发光探针，可用于检测内源性产生的超氧阴离子自由基在细胞的存在。

Benzenepentacarboxylic Acid (Pentacarboxybenzene) 是具有发现和清除HO 自由基作用的荧光染料。

DHBS (DCHBS) 与 4-氨基安替比林和过氧化氢联合使用，能够用于偶联酶促反应中过氧化物酶的显色定量。它是 Trinder 试剂的组分，能够与过氧化物酶一起测量过氧化氢的产生。

Dihydrofluorescein diacetate (Diacetyldihydrofluorescein) 是一种荧光探针，用于测量无细胞系统和细胞模型中的氧化应激。

Hydroxyphenyl Fluorescein (HPF) 是一种用于检测高活性氧的细胞渗透性荧光染料，几乎没有内在荧光。Hydroxyphenyl Fluorescein 可应用于荧光酶标仪和流式细胞仪，可用于研究神经退行性变和衰老。其荧光波长：Ex/Em=490/515 nm。

DAF-FM DA (Diaminofluorescein-FM diacetate) 是一种用于检测一氧化氮 (NO)和活性氮 （RNS） 水平的活性染料，可用于生物成像 (Ex/Em=495/515 nm)。

ROX dyes have a strong red fluorescence.Their excitation and emission wavelengths are longer than those of traditional rhodamine dyes.They are used as labels for peptides, proteins, and other biological ligands.5-ROX, 6-ROx, and their mixture of 5(6) -RoX

HKPerox-1 is a yellow-emitting fluorescent probe with excellent selectivity and sensitivity toward H 2 O 2 . HKPerox-1 can be used for molecular imaging of endogenous H 2 O 2 in living cells.

Resorufin acetate, a fluorescent dye, is used to selectively detect hydrazine [1].

CB2-H为一种高特异性双通道荧光探针，专门设计用于HOCl和ONOO-的同时检测。该探针在两个分离的通道中对HOCl和ONOO-无交叉干扰的检测能力使其适用于对活细胞和斑马鱼样本内HOCl及ONOO-的内源性水平进行双通道荧光成像分析，即使在不同生物刺激下也能保持准确性。

Peroxyfluor 1 是一种可渗透细胞的 H2O2 探针，属于第一代绿色荧光探针。

NIR-FP是一款用于超氧阴离子(O2-)检测的近红外荧光探针，具备高灵敏度和高选择性。由于O2-是活性氧种(ROS)的关键前体，此探针能有效动态监测由超氧阴离子引发的铁死亡介导的癫痫模型。这些模型包括红藻氨酸诱导的慢性癫痫模型、Pentylenetetrazole诱导的急性癫痫模型以及毛果芸香碱诱导的癫痫模型。

DPPP is a probe that reacts stoichiometrically with hydroperoxides to yield the fluorescent molecule diphenyl-1-pyrenylphosphine oxide (DPPP-O). Plasma levels of lipid hydroperoxides of phosphatidylcholine, phosphatidylethanolamine, triglycerides, and cholesteryl esters have been measured by HPLC with a post column detection system using DPPP. DPPP has also been used as a fluorescent probe for the detection of low density lipoprotein and cellular oxidation. Fluorescence of DPPP-O can be monitor using excitation and emission wavelengths of 351 nm and 380 nm, respectively.

5(6)-Carboxy-2’,7’-dichlorofluorescein diacetate is an oxidant-sensitive and cell-permeable fluorescent probe.1Base hydrolysis of the ester bonds by intracellular esterases releases the acetate groups and the resulting compound, 5(6)-carboxy-2’,7’,-dichlorofluorescein, is oxidized by reactive oxygen species (ROS) and nitric oxide (NO) and displays excitation emission maxima of 504 and 530 nm, respectively, which can be quantified as a measure of oxidant production. 1.Hempel, S.L., Buettner, G.R., O’Malley, Y.Q., et al.Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: Comparison with 2’,7’-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123Free Radic. Biol. Med.27(1-2)146-159(1999)

FHZ is a fluorescent probe.

BHHCT 是一种多功能染料，广泛应用于生物实验中，帮助研究人员观察和分析细胞结构、追踪生物分子、评估细胞功能、区分细胞类型、检测生物分子、研究组织病理及监测微生物，涵盖从基础科学研究到临床诊断的广泛领域。此外，染料在纺织品染色、功能性纺织品处理、食品色素和染料敏化太阳能电池等领域也有广泛应用。

HKOCl-4 (BXY2142) is a rhodol-based yellow fluorescent probe that demonstrates exceptional sensitivity and selectivity in detecting hypochlorous acid. Its absorption wavelength is significantly longer than that of fluorescein-based probes, and it exhibits better pH stability. Notably, HKOCl-4 exhibits excitation at 530 nm and emission at 557 nm.