Cross-linked dextran G 200 is a hydrophilic gel designed for the separation of macromolecules based on molecular size exclusion. It operates through a gel permeation mechanism; its cross-linked structure forms a three-dimensional network with specific pore sizes, facilitating separation according to the hydrodynamic volume of molecules. This compound is utilized to regulate the partition coefficient of permeating solutes and maintain osmotic balance, and is commonly applied in gel filtration chromatography for the purification and analysis of biomacromolecules like proteins and nucleic acids. Additionally, Cross-linked dextran G 200 serves as a gel filtration medium (particle size range: 40-120 μm; spherical protein separation range: 5k-600k Da).