Chondroitin Sulfate Methacryloyl (CSMA) is a methacryloyl-modified form of chondroitin sulfate known for its biocompatibility. It exhibits a higher degree of methacrylic substitution compared to HAMA (HY-158220), which is closely linked to customizable mechanical properties, swelling behavior, and enzymatic degradability. CSMA serves as a versatile biomaterial suitable for biomimetic hydrogel scaffolds and is an ideal hydrogel ink for 3D printing. Under the influence of photoinitiator LAP, it self-assembles into a fibrous hydrogel targeting bioactive adhesion sites, supporting tissue cell interaction and biodegradation. It is applicable in areas such as cell culture, biological 3D printing, and tissue engineering.