BP Fluor 532 NHS ester is an amine-reactive yellow fluorescent dye commonly employed for labeling proteins or antibodies through primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling process is most effective at pH 7-9, resulting in the formation of stable covalent amide bonds. BP Fluor 532 exhibits bright yellow fluorescence that is insensitive to pH changes from 4 to 10 and is optimally excited by the Nd:YAG laser line. It can bind with a variety of antibodies, peptides, proteins, tracers, and amplification substrates, and is frequently used to produce stable signals in imaging and flow cytometry.