Azimilide is a multi-ion-channel blocker that primarily inhibits fast (I_Kr, EC₅₀ = 0.39 µM) and slow (I_Ks, EC₅₀ = 0.59 µM) delayed rectifying potassium currents; at high concentrations, it blocks L-type calcium currents (EC₅₀ = 7.5 µM) and produces use-dependent sodium channel blockade. In canine ventricular myocytes, a concentration of 1 µM prolongs the action potential duration by 25% (at a stimulation frequency of 0.33 Hz), while a concentration of 5 µM produces a bidirectional modulatory effect. Additionally, this compound is commonly used to study repolarization dispersion and antiarrhythmic mechanisms under ischemia-reperfusion conditions.

DMSO: <1 mg/mL