Anti-ZNRF3 Polyclonal Antibody is a Rabbit antibody targeting ZNRF3. Anti-ZNRF3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ZNRF3) Polyclonal Antibody, Unconjugated (TMAB-14450) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample:
Lane 1: Human HepG2 cell Lysates
Lane 2: Human A549 cell Lysates
Lane 3: Human U-2 OS cell Lysates
Primary: Anti-ZNRF3 (TMAB-14450) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kDa
Observed band size: 120 kDa
3. 25 μg total protein per lane of various lysates (see on figure) probed with ZNRF3 polyclonal antibody, unconjugated (TMAB-14450) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:50-200

Polyclonal

KLH conjugated synthetic peptide: human ZNRF3

Human

ZNRF3

E3 ubiquitin-protein ligase ZNRF3

E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone.