Anti-ZNF385B Polyclonal Antibody is a Rabbit antibody targeting ZNF385B. Anti-ZNF385B Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Sheep)

1. Blank control: 293T.
Primary Antibody (green line): Rabbit Anti-ZNF385B antibody (TMAB-14374)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample:
Spleen (Mouse) Lysate at 40 μg
Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-ZNF385B (TMAB-14374) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD

WB: 1:500-2000; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human ZNF385B

Human

ZNF385B

Zinc finger protein 385B

May play a role in p53/TP53-mediated apoptosis.