Anti-ZNF185 Polyclonal Antibody is a Rabbit antibody targeting ZNF185. Anti-ZNF185 Polyclonal Antibody can be used in WB,FCM.

IgG

Human (predicted:Mouse,Rat,Dog)

1. Sample:
MCF-7 (Human) Cell Lysate at 40 μg
A431 (Human) Cell Lysate at 40 μg
U2os (Human) Cell Lysate at 40 μg
Primary: Anti-ZNF185 (TMAB-14340) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 76/39 kD
Observed band size: 67 kD
2. Blank control: A431.
Primary Antibody (green line): Rabbit Anti-Phospho-c-Fos (Ser32) antibody
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 3μg/Test

Polyclonal

KLH conjugated synthetic peptide: human ZNF185

Human

ZNF185

Zinc finger protein 185

May be involved in the regulation of cellular proliferation and/or differentiation.