Anti-YKL-40/CHI3L1 Polyclonal Antibody 2 is a Rabbit antibody targeting YKL-40/CHI3L1. Anti-YKL-40/CHI3L1 Polyclonal Antibody 2 can be used in WB,ICC/IF.

IgG

Human,Mouse,Rat

1. U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (CHI3L1) polyclonal Antibody, Unconjugated (TMAB-00426) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
2. Protein:
Liver (Rat) lysate at 30 μg;
Colon carcinoma (Human) lysates at 30 μg;
Primary: Anti-CGP-39/CHI3L1/YKL-40 (TMAB-00426) at 1:200;
Secondary: HRP conjugated Goat Anti-Rabbit Igg (secondary antibody) at 1: 3000;
Predicted band size: 42 kDa
Observed band size: 39 kDa
3. Sample:
Lane 1: Mouse Lung tissue lysates
Lane 2: Rat Lung tissue lysates
Primary: Anti-CHI3L1 (TMAB-00426) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa

WB: 1:500-2000; ICC/IF: 1:100

Polyclonal

KLH conjugated synthetic peptide: rat CHI3L1

Rat

Other ECM Proteins