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Anti-WISP2/CCN5 Polyclonal Antibody

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Anti-WISP2/CCN5 Polyclonal Antibody
产品编号 TMAB-01980

Anti-WISP2/CCN5 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 WISP2/CCN5。Anti-WISP2/CCN5 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-WISP2/CCN5 Polyclonal Antibody

Anti-WISP2/CCN5 Polyclonal Antibody

Rating icon 还可以
Anti-WISP2/CCN5 Polyclonal Antibody
产品编号 TMAB-01980

Anti-WISP2/CCN5 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 WISP2/CCN5。Anti-WISP2/CCN5 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,165
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100 μL
¥ 1,960
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200 μL
¥ 2,790
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产品介绍

生物活性
产品描述
Anti-WISP2/CCN5 Polyclonal Antibody is a Rabbit antibody targeting WISP2/CCN5. Anti-WISP2/CCN5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Human,Mouse (predicted:Rat,Pig,Cow,Rabbit,Sheep)
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (WISP2) Polyclonal Antibody, Unconjugated (TMAB-01980) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample: A431 Cell (Human) Lysate at 40 μg
Primary: Anti-WISP2 (TMAB-01980) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
3. Sample: A549 Cell (Human) Lysate at 40 μg
Primary: Anti-WISP2 (TMAB-01980) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Secreted.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景WISP2 a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. WISP2 lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover.
抗原信息
免疫原
KLH conjugated synthetic peptide: human CTGFL
抗原种属
Human
基因ID
Uniprot ID
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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