Anti-Whitlow linker Antibody (1M598) is a Mouse antibody targeting Whitlow linker. Anti-Whitlow linker Antibody (1M598) can be used in ELISA, WB, FC.

IgG2b

1M598

Human

1. The Binding Activity of Whitlow linker with Anti-Whitlow linker antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized scFv-based Anti-CD19 CAR recombinant antibody at 2 μg/mL can bind Anti-Whitlow linker antibody. The EC50 is 5.147 to 5.761 ng/mL.
2. Western Blot
-Positive WB detected in: 293F whole cell lysate, scFv-based Anti-CD19 CAR containing a Whitlow linker (293F/CD19 CAR) whole cell lysate
-All lanes: Whitlow linker antibody at 1:1000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 50-200 kDa
-Observed band size: 50-200 KDa
-Exposure time：5s
3. Untransfected HEK-293F cells (green line) and transfected scFv-based Anti-CD19 CAR containing a Whitlow/218 linker stable cells (red line) were stained with anti-Whitlow linker antibody (2µg/1*10^6 cells), washed and then followed by FITC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry.

ELISA, WB, FC

WB:1:1000-1:5000; FC:1:50-1:200.

Monoclonal

Unconjugated

FITC

2

Whitlow linker-KLH

Others