Anti-VSIG4 Polyclonal Antibody is a Rabbit antibody targeting VSIG4. Anti-VSIG4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Mouse,Rat (predicted:Human,Dog,Cow)

1. Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-VSIG4 Polyclonal Antibody, Unconjugated (TMAB-01974) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lung (Mouse) lysate at 30 μg
Placenta (Mouse) Lysate at 30 μg
Primary: Anti-VSIG4 (TMAB-01974) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
3. Sample:
Placenta (Mouse) Lysate at 40 μg
Primary: Anti-VSIG4 (TMAB-01974) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
4. Sample:
Lung (Mouse) Lysate at 40 μg
Primary: Anti-VSIG4 (TMAB-01974) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human VSIG4

Human

Receptors,Classical Pathway,Macrophage / Inflamm.