Anti-Von Willebrand Factor/vWF Polyclonal Antibody is a Rabbit antibody targeting Von Willebrand Factor/vWF. Anti-Von Willebrand Factor/vWF Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit)

1. Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-VWF Polyclonal Antibody, Unconjugated (TMAB-01972) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus.
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-VWF Polyclonal Antibody, Unconjugated (TMAB-01972) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (VWF) Polyclonal Antibody, Unconjugated (TMAB-01972) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human von Willebrand antigen 2

Human

Angiogenic growth factors,Endothelial Cell Markers,Extrinsic,Regulatory,Platelets,Serum Proteins,Endothelial Markers