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Anti-Vitamin D Receptor/VDR Antibody (5I578)

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产品编号 TMAH-01244

Anti-Vitamin D Receptor/VDR Antibody (5I578) 是一种抗体,靶向 Vitamin D Receptor/VDR。Anti-Vitamin D Receptor/VDR Antibody (5I578) 可用于 ELISA, WB, IHC。

Anti-Vitamin D Receptor/VDR Antibody (5I578)

Anti-Vitamin D Receptor/VDR Antibody (5I578)

Rating icon 还可以
产品编号 TMAH-01244

Anti-Vitamin D Receptor/VDR Antibody (5I578) 是一种抗体,靶向 Vitamin D Receptor/VDR。Anti-Vitamin D Receptor/VDR Antibody (5I578) 可用于 ELISA, WB, IHC。

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产品介绍

生物活性
产品描述
Anti-Vitamin D Receptor/VDR Antibody (5I578) is an antibody targeting Vitamin D Receptor/VDR. Anti-Vitamin D Receptor/VDR Antibody (5I578) can be used in ELISA, WB, IHC.
Ig Type
Rabbit IgG
克隆号
5I578
交叉反应
Human
验证活性
1. Western Blot
-Positive WB detected in: MCF-7 whole cell lysate, THP-1 whole cell lysate, HL-60 whole cell lysate
-All lanes: Vitamin D antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 49, 54 kDa
-Observed band size: 49 kDa
2. IHC image of TMAH-01244 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
应用
ELISA, WB, IHC
推荐剂量
WB:1:500-1:5000; IHC:1:50-1:200.
抗体种类
Monoclonal
亚细胞定位Nucleus. Cytoplasm.
构建方式Recombinant Antibody
纯化方式Affinity-chromatography
性状Liquid
缓冲液Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells. Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. Plays a central role in calcium homeostasis.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthesized peptide derived from human Vitamin D Receptor
抗原种属
Human
基因ID
7421
Uniprot ID
研究领域
Epigenetics and Nuclear Signaling, Cancer, Signal transduction
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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