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Anti-Vitamin D Receptor/VDR Antibody (5I578) 是一种抗体,靶向 Vitamin D Receptor/VDR。Anti-Vitamin D Receptor/VDR Antibody (5I578) 可用于 ELISA, WB, IHC。
Anti-Vitamin D Receptor/VDR Antibody (5I578) 是一种抗体,靶向 Vitamin D Receptor/VDR。Anti-Vitamin D Receptor/VDR Antibody (5I578) 可用于 ELISA, WB, IHC。
产品描述 | Anti-Vitamin D Receptor/VDR Antibody (5I578) is an antibody targeting Vitamin D Receptor/VDR. Anti-Vitamin D Receptor/VDR Antibody (5I578) can be used in ELISA, WB, IHC. |
Ig Type | Rabbit IgG |
克隆号 | 5I578 |
交叉反应 | Human |
验证活性 | 1. Western Blot -Positive WB detected in: MCF-7 whole cell lysate, THP-1 whole cell lysate, HL-60 whole cell lysate -All lanes: Vitamin D antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 49, 54 kDa -Observed band size: 49 kDa 2. IHC image of TMAH-01244 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
应用 | ELISA, WB, IHC |
推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200. |
抗体种类 | Monoclonal |
亚细胞定位 | Nucleus. Cytoplasm. |
构建方式 | Recombinant Antibody |
纯化方式 | Affinity-chromatography |
性状 | Liquid |
缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells. Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. Plays a central role in calcium homeostasis. |
偶联 | Unconjugated |
免疫原 | A synthesized peptide derived from human Vitamin D Receptor |
抗原种属 | Human |
基因ID | 7421 |
Uniprot ID | |
研究领域 | Epigenetics and Nuclear Signaling, Cancer, Signal transduction |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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