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Anti-Vimentin Antibody (5Y646)
还可以产品编号 TMAY-00097
Anti-Vimentin Antibody (5Y646) 是一种 Rabbit 抗体，靶向 Vimentin。Anti-Vimentin Antibody (5Y646) 可用于 WB,IHC-P。
Anti-Vimentin Antibody (5Y646)
Anti-Vimentin Antibody (5Y646)
还可以产品编号 TMAY-00097
Anti-Vimentin Antibody (5Y646) 是一种 Rabbit 抗体，靶向 Vimentin。Anti-Vimentin Antibody (5Y646) 可用于 WB,IHC-P。
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抗原信息
| 产品描述 | Anti-Vimentin Antibody (5Y646) is a Rabbit antibody targeting Vimentin. Anti-Vimentin Antibody (5Y646) can be used in WB,IHC-P. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 5Y646 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-VIM rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: A549 Whole Cell Lysate. -Lane C: 293T Whole Cell Lysate. -Lane D: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:54 kDa. -Observed band size:55 kDa. 2. Immunochemical staining of human Vimentin in human stomach with rabbit monoclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections. 3. Immunochemical staining of human Vimentin in human malignant melanoma with rabbit monoclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections. 4. Immunochemical staining of human Vimentin in human lung cancer with rabbit monoclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections. |
| 应用 | WB,IHC-P |
| 推荐剂量 | WB: 1:500-1:2000; IHC-P: 1:1000-1:10000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human Vimentin/VIM. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Vimentin is a type III intermediate filament (IF) protein found in various non-epithelial cells, especially mesenchymal cells. A vimentin monomer, has a central α-helical domain and carboxyl (tail) domains. Two monomers compose the basic subunit of vimentin assembly. Vimentin is crucial for supporting and anchoring the position of the organelles in the cytosol. Vimentin provided cells with a resilience absent from the microtubule or actin filament networks, when under mechanical stress in vivo. Therefore, in general, it is accepted that vimentin is the cytoskeletal component responsible for maintaining cell integrity. Vimentin is also responsible for stabilizing cytoskeletal interactions. It is found that vimentin control the transport of low-density lipoprotein. It has been used as a sarcoma tumor marker to identify mesenchyme. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide corresponding to the center region of the Human Vimentin/VIM |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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