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Anti-VEGFR1/FLT-1 Polyclonal Antibody
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产品编号 TMAB-01955
Anti-VEGFR1/FLT-1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 VEGFR1/FLT-1。Anti-VEGFR1/FLT-1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-VEGFR1/FLT-1 Polyclonal Antibody
Anti-VEGFR1/FLT-1 Polyclonal Antibody
还可以产品编号 TMAB-01955
Anti-VEGFR1/FLT-1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 VEGFR1/FLT-1。Anti-VEGFR1/FLT-1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,160 | 5日内发货 | |
| 100 μL | ¥ 1,970 | 5日内发货 | |
| 200 μL | ¥ 2,790 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-VEGFR1/FLT-1 Polyclonal Antibody is a Rabbit antibody targeting VEGFR1/FLT-1. Anti-VEGFR1/FLT-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (VEGFR1) Polyclonal Antibody, Unconjugated (TMAB-01955) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: Recombinant human VEGFR1 protein, C-His (HEK293) Primary: Anti-VEGFR1 (TMAB-01955) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 147 kDa Observed band size: 140 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:400-800; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Isoform 1: Cell membrane; Single-pass type I membrane protein. Endosome. Note=Autophosphorylation promotes ubiquitination and endocytosis；Isoform 2: Secreted；Isoform 3: Secreted；Isoform 4: Secreted；Isoform 5: Cytoplasm (Potential)；Isoform 6: Cytoplasm (Potential)；Isoform 7: Cytoplasm (Potential). |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | VEGF Receptor 1 (also known as FLT) belongs to the src gene family and shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The protein acts as a receptor for VEGF, VEGFB and PGF. An alternatively spliced form of the gene produces a soluble protein (sFlt1) which binds vascular endothelial growth factor (VEGF) with high affinity. sFlt1 has a higher affinity for VEGF indicating that it may function as an inhibitor in the VEGF response. VEGF Receptor 1 is specifically expressed in most vascular endothelial cells and peripheral blood monocytes. |
| 免疫原 | KLH conjugated synthetic peptide: mouse VEGFR1 |
| 抗原种属 | Mouse |
| 研究领域 | Response to hypoxia,VEGF,Angiogenic growth factors,Growth factors,Endothelial Cell Markers,VEGF Receptors,Endothelium,Hypoxia,Cancer,VEGF,Tyrosine Kinases |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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