Anti-VEGFR1/FLT-1 Polyclonal Antibody is a Rabbit antibody targeting VEGFR1/FLT-1. Anti-VEGFR1/FLT-1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human

1. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (VEGFR1) Polyclonal Antibody, Unconjugated (TMAB-01955) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Lane 1: Recombinant human VEGFR1 protein, C-His (HEK293)
Primary: Anti-VEGFR1 (TMAB-01955) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 147 kDa
Observed band size: 140 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:400-800; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: mouse VEGFR1

Mouse

FLT1

Vascular endothelial growth factor receptor 1

Response to hypoxia,VEGF,Angiogenic growth factors,Growth factors,Endothelial Cell Markers,VEGF Receptors,Endothelium,Hypoxia,Cancer,VEGF,Tyrosine Kinases

Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.