Anti-VEGFA Polyclonal Antibody 5 is a Rabbit antibody targeting VEGFA. Anti-VEGFA Polyclonal Antibody 5 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Rat (predicted:Dog)

1. Sample:
Cerebellum (Rat) Lysate at 40 μg
Kidney (Rat) Lysate at 40 μg
Cerebrum (Rat) Lysate at 40 μg
Liver (Rat) Lysate at 40 μg
Primary: Anti-VEGFA (TMAB-01952) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43/23 kDa
Observed band size: 23 kDa
2. Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (VEGFA) Polyclonal Antibody, Unconjugated (TMAB-01952) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (VEGFA) Polyclonal Antibody, Unconjugated (TMAB-01952) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Sample:
Lane 1: Human K562 cell lysates
Lane 2: Human MDA-MB-231 cell lysates
Primary: Anti-VEGFA (TMAB-01952) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human VEGFA

Human

VEGF,SARS Coronavirus,Cancer,Hypoxia,Co-factors,Angiogenesis and vasculogenesis,VEGF,Angiogenic growth factors,VEGF,Response to hypoxia