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Anti-VEGFA Polyclonal Antibody 3 是一种 Rabbit 抗体,靶向 VEGFA。Anti-VEGFA Polyclonal Antibody 3 可用于 WB,IHC-P,IHC-Fr,IF,ELISA。
Anti-VEGFA Polyclonal Antibody 3 是一种 Rabbit 抗体,靶向 VEGFA。Anti-VEGFA Polyclonal Antibody 3 可用于 WB,IHC-P,IHC-Fr,IF,ELISA。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,165 | 5日内发货 | |
100 μL | ¥ 1,975 | 5日内发货 | |
200 μL | ¥ 2,780 | 5日内发货 |
产品描述 | Anti-VEGFA Polyclonal Antibody 3 is a Rabbit antibody targeting VEGFA. Anti-VEGFA Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,IF,ELISA. |
别名 | VPF, VEGF-A, VEGF, MVCD1, L-VEGF |
Ig Type | IgG |
交叉反应 | Human (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep) |
验证活性 | Tissue/cell: human brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-VEGF Polyclonal Antibody, Unconjugated (TMAB-01951) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. |
组织特异性 | soform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. |
构建方式 | Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
浓度 | 1 mg/mL |
研究背景 | Vascular endothelial growth factor (VEGF), originally known as vascular permeability factor (VPF), is a signal protein produced by cells that stimulates the formation of blood vessels. To be specific, VEGF is a sub-family of growth factors, the platelet-derived growth factor family of cystine-knot growth factors. They are important signaling proteins involved in both vasculogenesis (the de novo formation of the embryonic circulatory system) and angiogenesis (the growth of blood vessels from pre-existing vasculature). |
免疫原 | KLH conjugated synthetic peptide: human VEGF |
抗原种属 | Human |
基因名称 | VEGFA |
基因ID | |
蛋白名称 | Vascular endothelial growth factor A |
Uniprot ID | |
研究领域 | VEGF,SARS Coronavirus,Cancer,Hypoxia,Co-factors,Angiogenesis and vasculogenesis,VEGF,Angiogenic growth factors,VEGF,Response to hypoxia |
功能 | Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. |
分子量 | Theoretical: 24 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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