Anti-VDAC3 Polyclonal Antibody is a Rabbit antibody targeting VDAC3. Anti-VDAC3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-VDAC3 Polyclonal Antibody, Unconjugated (TMAB-01944) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Large intestine (Rat) Lysate at 40 μg
Primary: Anti-VDAC3 (TMAB-01944) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
3. Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Mouse Spleen tissue lysates
Lane 3: Rat Kidney tissue lysates
Lane 4: Rat Spleen tissue lysates
Primary: Anti-VDAC3 (TMAB-01944) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human VDAC3

Human