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Anti-V5 Tag Antibody (4I665) 是一种 Mouse 抗体,靶向 V5 Tag。Anti-V5 Tag Antibody (4I665) 可用于 WB。
Anti-V5 Tag Antibody (4I665) 是一种 Mouse 抗体,靶向 V5 Tag。Anti-V5 Tag Antibody (4I665) 可用于 WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 20 μL | ¥ 780 | 5日内发货 | |
| 100 μL | ¥ 1,585 | 5日内发货 | |
| 500 μL | ¥ 3,985 | 5日内发货 |
| 产品描述 | Anti-V5 Tag Antibody (4I665) is a Mouse antibody targeting V5 Tag. Anti-V5 Tag Antibody (4I665) can be used in WB. |
| Ig Type | Mouse IgG1 |
| 克隆号 | 4I665 |
| 反应种属 | other |
| 特异性 | Recognize N-terminal and C-terminal V5 Tag in fusion proteins |
| 验证活性 | Anti-V5 Tag mouse monoclonal antibody at 1:500 dilution. -Lane A: his-ARG1-V5 transfected 293 cell Lysate(60ug) -Lane B: mFABP4-V5-his transfected 293 cell Lysate(60ug) -Lane C: V5-ARG1-his transfected 293 cell Lysate(10ug) -Lane D: V5-mFABP4-his transfected 293 cell Lysate(20ug) -Lane E: Negative control transfected 293 cell Lysate(30ug) -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. |
| 应用 | WB |
| 推荐剂量 | WB: 1:500-1:2000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | The V5 epitope tag has a 14-amino-acid sequence (GKPIPNPLLGLDST) which is derived from simian parainfluenza virus type 5 (SV5). The V5 epitope tag is used to label a protein of interest so that the tagged protein can be analyzed and visualized. Anti-V5 epitope tag antibody products recognize and detect V5-tagged proteins without a protein-specific antibody or probe in several immunochemical assays such as Western Blotting, ELISAs and immunostaining techniques. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: V5 tag sequence (GKPIPNPLLGLDST). |
| 抗原种属 | other |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
| 存储 | -20°C for 1 year |