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Anti-UQCRC2 Antibody (7Z907)
还可以产品编号 TMAH-01235
别名 UQCRC 2, Ubiquinol-cytochrome-c reductase complex core protein 2, Cytochrome b-c1 complex subunit 2, Core protein II, Complex III subunit 2
Anti-UQCRC2 Antibody (7Z907) 是一种抗体，靶向 UQCRC2。Anti-UQCRC2 Antibody (7Z907) 可用于 ELISA, WB, IHC。
Anti-UQCRC2 Antibody (7Z907)
Anti-UQCRC2 Antibody (7Z907)
还可以产品编号 TMAH-01235 别名 UQCRC 2, Ubiquinol-cytochrome-c reductase complex core protein 2, Cytochrome b-c1 complex subunit 2, Core protein II, Complex III subunit 2
Anti-UQCRC2 Antibody (7Z907) 是一种抗体，靶向 UQCRC2。Anti-UQCRC2 Antibody (7Z907) 可用于 ELISA, WB, IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,300 | 5日内发货 | |
| 100 μL | ¥ 2,195 | 5日内发货 |
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产品介绍
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抗原信息
| 产品描述 | Anti-UQCRC2 Antibody (7Z907) is an antibody targeting UQCRC2. Anti-UQCRC2 Antibody (7Z907) can be used in ELISA, WB, IHC. |
| 别名 | UQCRC 2, Ubiquinol-cytochrome-c reductase complex core protein 2, Cytochrome b-c1 complex subunit 2, Core protein II, Complex III subunit 2 |
| Ig Type | Rabbit IgG |
| 克隆号 | 7Z907 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in:HepG2 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate -All lanes: UQCRC2 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 49 kDa -Observed band size: 49 kDa 2. IHC image of TMAH-01235 diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. IHC image of TMAH-01235 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| 应用 | ELISA, WB, IHC |
| 推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. The 2 core subunits UQCRC1/QCR1 and UQCRC2/QCR2 are homologous to the 2 mitochondrial-processing peptidase (MPP) subunits beta-MPP and alpha-MPP respectively, and they seem to have preserved their MPP processing properties. May be involved in the in situ processing of UQCRFS1 into the mature Rieske protein and its mitochondrial targeting sequence (MTS)/subunit 9 when incorporated into complex III (Probable). |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human UQCRC2 |
| 抗原种属 | Human |
| 基因ID | 7385 |
| Uniprot ID | |
| 研究领域 | Cancer, Metabolism, Signal transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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