Anti-UCP2 Polyclonal Antibody is a Rabbit antibody targeting UCP2. Anti-UCP2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Pig,Horse,Rabbit)

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (UCP-2) Polyclonal Antibody, Unconjugated (TMAB-01931) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Lane 1: Stomach (Rat) Lysate at 40 μg
Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 3: SiHa (Human) Cell Lysate at 30 μg
Lane 4: DU145 (Human) Cell Lysate at 30 μg
Lane 5: RAW264.7 (Mouse) Cell Lysate at 30 μg
Lane 6: Liver (Mouse) Lysate at 40 μg
Primary: Anti-UCP-2 (TMAB-01931) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
3. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (UCP-2) polyclonal Antibody, Unconjugated (TMAB-01931) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
4. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (UCP-2) polyclonal Antibody, Unconjugated (TMAB-01931) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
5. Blank control (blue line): Hela (blue).
Primary Antibody (green line): Rabbit Anti-UCP-2 antibody (TMAB-01931)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): f (ab/)2 fragment goat anti-rabbit IgG-FITC
Dilution: 1 μg/test.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 1μg /test

Polyclonal

KLH conjugated synthetic peptide: mouse UCP-2

Mouse

Integration of energy metabolism,Integration of energy,Cancer,Obesity