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Anti-Ubiquitin D Antibody (7G418)
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产品编号 TMAC-04189
别名 Ubiquitin-like protein FAT10, UbiquitinD, Ubiquitin like protein FAT10, UBD_HUMAN, UBD 3, Ubd, GABBR1, FAT10, Diubiquitin
Anti-Ubiquitin D Antibody (7G418) 是一种 Rabbit 抗体，靶向 Ubiquitin D。Anti-Ubiquitin D Antibody (7G418) 可用于 WB,ICC,IHC。
Anti-Ubiquitin D Antibody (7G418)
Anti-Ubiquitin D Antibody (7G418)
还可以产品编号 TMAC-04189 别名 Ubiquitin-like protein FAT10, UbiquitinD, Ubiquitin like protein FAT10, UBD_HUMAN, UBD 3, Ubd, GABBR1, FAT10, Diubiquitin
Anti-Ubiquitin D Antibody (7G418) 是一种 Rabbit 抗体，靶向 Ubiquitin D。Anti-Ubiquitin D Antibody (7G418) 可用于 WB,ICC,IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
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| 产品描述 | Anti-Ubiquitin D Antibody (7G418) is a Rabbit antibody targeting Ubiquitin D. Anti-Ubiquitin D Antibody (7G418) can be used in WB,ICC,IHC. |
| 别名 | Ubiquitin-like protein FAT10, UbiquitinD, Ubiquitin like protein FAT10, UBD_HUMAN, UBD 3, Ubd, GABBR1, FAT10, Diubiquitin |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse |
| 验证活性 | 1. Western blot analysis of Ubiquitin D on Hela cells lysates using anti-Ubiquitin D antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Ubiquitin D antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Ubiquitin D antibody. Counter stained with hematoxylin. 4. ICC staining Ubiquitin D in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Ubiquitin D in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Ubiquitin D in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:100-500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | FAT10, also designated Ubiquitin D or Diubiquitin, is a 165 amino acid protein encoded in the major histocompatibility complex (MHC) that consists of two domains which share significant homology with ubiquitin. Each domain contains two cysteines, along with a free C-terminal diglycine motif required for FAT10 conjugate formation. FAT10 is inducible by interferon-g and tumor necrosis factor a (TNF?). The FAT10 protein interacts with MAD2, a component of the spindle checkpoint, and plays a role in antigen presentation, cytokine response, apoptosis and mitosis. It may also regulate cell growth during dendritic cell or B cell activation and development. FAT10 mRNA is expressed mainly in some dendritic cells and lymphoblastoid lines and in other specific cells subsequent to interferon-g induction. The human FAT10 gene, designated UBD, maps to chromosome 6p21.3 and is overexpressed in the tumors of various epithelial cancers. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 18 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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