Anti-UBE2V1 Polyclonal Antibody is a Rabbit antibody targeting UBE2V1. Anti-UBE2V1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Rat (predicted:Mouse,Chicken,Pig,Horse,Sheep)

1. Blank control: K562.
Primary Antibody (green line): Rabbit Anti-UBE2V1 antibody (TMAB-13944)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Paraformaldehyde-fixed, paraffin embedded (human thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (UBE2V1) Polyclonal Antibody, Unconjugated (TMAB-13944) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Blank control: K562.
Primary Antibody (green line): Rabbit Anti-UBE2V1 antibody (TMAB-13944)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
4. Paraformaldehyde-fixed, paraffin embedded (rat thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (UBE2V1) Polyclonal Antibody, Unconjugated (TMAB-13944) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human UBE2V1

Human

UBE2V1

Ubiquitin-conjugating enzyme E2 variant 1

Has no ubiquitin ligase activity on its own. TheUBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonicalpoly-ubiquitin chains that are linked through Lys-63. This type ofpoly-ubiquitination activates IKK and does not seem to involveprotein degradation by the proteasome. Plays a role in theactivation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2.Mediates transcriptional activation of target genes. Plays a rolein the control of progress through the cell cycle anddifferentiation. Plays a role in the error-free DNA repair pathwayand contributes to the survival of cells after DNA damage.