Anti-TWIST Polyclonal Antibody is a Rabbit antibody targeting TWIST. Anti-TWIST Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human)

1. Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TWIST) Polyclonal Antibody, Unconjugated (TMAB-01921) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TWIST) Polyclonal Antibody, Unconjugated (TMAB-01921) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human TWIST

Human

TWIST1

Twist-related protein 1

Transcription factors,Transcription factors/regulators,Mesoderm,Muscle,HLH,Soma marker,Mesoderm

Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.