Anti-TSG101 Polyclonal Antibody is a Rabbit antibody targeting TSG101. Anti-TSG101 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Rat (predicted:Mouse,Dog,Cow,Horse,Rabbit)

1. Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Tsg101 Polyclonal Antibody, Unconjugated (TMAB-01910) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Tsg101 Polyclonal Antibody, Unconjugated (TMAB-01910) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Tsg101 Polyclonal Antibody, Unconjugated (TMAB-01910) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Sample:
Lane1: Brain (Rat) Lysate at 30 μg
Lane2: Heart (Rat) Lysate at 30 μg
Primary: Anti-Tsg101 (TMAB-01910) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit Igg (bse-0295G) at 1: 3000 dilution;
Predicted band size: 43 kDa
Observed band size: 43 kDa
5. Sample:
Lane 1: MCF-7 (Human) Cell Lysate at 30 μg
Primary:
Anti-TSG101 (TMAB-01910) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
6. Sample:
Lane 1: SH-SY5Y (Human) Cell Lysate at 30 μg
Lane 2: 293T (Human) Cell Lysate at 30 μg
Lane 3: HepG2 (Human) Cell Lysate at 30 μg
Lane 4: A549 (Human) Cell Lysate at 30 μg
Primary: Anti-Tsg101 (TMAB-01910) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Tsg101

Human

Cell cycle inhibitors,Cell differentiation,Cell Cycle Inhibitors,Cell Cycle Inhibitors,Cell Differentiation,Host Virus Interaction