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Anti-TSG101 Polyclonal Antibody

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货号 TMAB-01910

Anti-TSG101 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 TSG101。Anti-TSG101 Polyclonal Antibody 可用于 IF,IHC-Fr,WB。

Anti-TSG101 Polyclonal Antibody

Anti-TSG101 Polyclonal Antibody

一键复制产品信息
Rating icon 还可以
货号 TMAB-01910

Anti-TSG101 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 TSG101。Anti-TSG101 Polyclonal Antibody 可用于 IF,IHC-Fr,WB。

规格价格库存数量
50 μL
¥ 1,175
5日内发货
100 μL
¥ 1,970
5日内发货
200 μL
¥ 2,785
5日内发货
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产品介绍


生物活性
产品描述
Anti-TSG101 Polyclonal Antibody is a Rabbit antibody targeting TSG101. Anti-TSG101 Polyclonal Antibody can be used in IF,IHC-Fr,WB.
Ig Type
IgG
反应种属
Human,Rat (predicted:Mouse,Dog,Cow,Horse,Rabbit)
验证活性
1. Sample:
Lane1: Brain (Rat) Lysate at 30 μg
Lane2: Heart (Rat) Lysate at 30 μg
Primary: Anti-Tsg101 (TMAB-01910) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit Igg (bse-0295G) at 1: 3000 dilution;
Predicted band size: 43 kDa
Observed band size: 43 kDa
2. Sample:
Lane 1: MCF-7 (Human) Cell Lysate at 30 μg
Primary:
Anti-TSG101 (TMAB-01910) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
3. Sample:
Lane 1: SH-SY5Y (Human) Cell Lysate at 30 μg
Lane 2: 293T (Human) Cell Lysate at 30 μg
Lane 3: HepG2 (Human) Cell Lysate at 30 μg
Lane 4: A549 (Human) Cell Lysate at 30 μg
Primary: Anti-Tsg101 (TMAB-01910) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
verifiedActivityverifiedActivityverifiedActivity
应用IFIHC-FrWB
推荐剂量
WB: 1:500-2000; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cytoplasm. Membrane; Peripheral membrane protein. Nucleus. Late endosome membrane; Peripheral membrane protein. Note=Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.
组织特异性Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1 mg/mL
研究背景The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008]
抗原信息
免疫原
KLH conjugated synthetic peptide: human Tsg101
抗原种属
Human
基因名称
Tsg101
基因ID
蛋白名称
Tumor susceptibility gene 101 protein
Uniprot ID
研究领域
Cell cycle inhibitors,Cell differentiation,Cell Cycle Inhibitors,Cell Cycle Inhibitors,Cell Differentiation,Host Virus Interaction
功能
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.
化学信息
分子量Theoretical: 44 kDa.
储存&溶解度
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储store at low temperature | store at -20°C

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