Anti-TRPC1 Polyclonal Antibody is a Rabbit antibody targeting TRPC1. Anti-TRPC1 Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit)

Blank control: A549.
Primary Antibody (green line): Rabbit Anti-TRPC1 antibody (TMAB-13837)
Dilution: 3 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 3μg/Test

Polyclonal

KLH conjugated synthetic peptide: human TRPC1

Human

TRPC1

Short transient receptor potential channel 1

Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Seems to be also activated by intracellular calcium store depletion.