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Anti-TRIM7 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 TRIM7。Anti-TRIM7 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-TRIM7 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 TRIM7。Anti-TRIM7 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,175 | 5日内发货 | |
100 μL | ¥ 1,975 | 5日内发货 | |
200 μL | ¥ 2,780 | 5日内发货 |
产品描述 | Anti-TRIM7 Polyclonal Antibody is a Rabbit antibody targeting TRIM7. Anti-TRIM7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Human (predicted:Mouse,Rat,Cow,Horse,Sheep) |
验证活性 | 1. Sample: Siha Cell (Human) Lysate at 40 μg Primary: Anti-TRIM7 (TMAB-01899) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TRIM7) Polyclonal Antibody, Unconjugated (TMAB-01899) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:50-200 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | The tripartite motif (TRIM) family of proteins are characterized by a conserved TRIM domain that includes a coiled-coil region, a B-box type zinc finger, one RING finger and three zinc-binding domains. TRIM7 (tripartite motif-containing 7), also known as RNF90 or GNIP, is a 511 amino acid protein that belongs to the TRIM family and contains one RING-type zinc finger, one B box-type zinc finger and one SPRY domain. Expressed in placenta and skeletal muscle and present at lower levels in brain, heart and pancreas, TRIM7 localizes to both the cytoplasm and the nucleus where it exists as dimers and is thought to participate in the initiation of glycogen synthesis. Multiple isoforms of TRIM7 exist due to alternative splicing events. |
免疫原 | KLH conjugated synthetic peptide: human TRIM7/RNF90 |
抗原种属 | Human |
基因ID | |
Uniprot ID |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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