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Anti-TRAP1/HSP75 Antibody (9U137)

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货号 TMAB-13742

Anti-TRAP1/HSP75 Antibody (9U137) 是一种 Rabbit 抗体，靶向 TRAP1/HSP75。Anti-TRAP1/HSP75 Antibody (9U137) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。

Anti-TRAP1/HSP75 Antibody (9U137)

Anti-TRAP1/HSP75 Antibody (9U137)

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Rating icon 还可以
货号 TMAB-13742

Anti-TRAP1/HSP75 Antibody (9U137) 是一种 Rabbit 抗体，靶向 TRAP1/HSP75。Anti-TRAP1/HSP75 Antibody (9U137) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。

规格价格库存数量
25 μL
¥ 795
5日内发货
50 μL
¥ 1,390
5日内发货
100 μL
¥ 2,485
5日内发货
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产品介绍

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产品描述
Anti-TRAP1/HSP75 Antibody (9U137) is a Rabbit antibody targeting TRAP1/HSP75. Anti-TRAP1/HSP75 Antibody (9U137) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Ig Type
IgG
克隆号
9U137
反应种属
Human
验证活性
1. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TRAP1/HSP75 Monoclonal Antibody, Unconjugated (TMAB-13742) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TRAP1/HSP75 Monoclonal Antibody, Unconjugated (TMAB-13742) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TRAP1/HSP75 Monoclonal Antibody, Unconjugated (TMAB-13742) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. 25 μg total protein per lane of various lysates (see on figure) probed with TRAP1/HSP75 monoclonal antibody, unconjugated (TMAB-13742) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
应用WBIHC-PIHC-FrICC/IFIF
推荐剂量
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200
抗体种类
Monoclonal
宿主来源Rabbit
亚细胞定位Mitochondrion.
组织特异性Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.
构建方式Recombinant Antibody
纯化方式Protein A purified
性状Liquid
缓冲液10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
研究背景Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, downstream of PINK1 and mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA.
抗原信息
免疫原
A synthesized peptide: human Hsp75
抗原种属
Human
基因名称
TRAP1
基因ID
10131
蛋白名称
Heat shock protein 75 kDa, mitochondrial
Uniprot ID
Q12931
功能
Chaperone that expresses an ATPase activity.
化学信息
分子量Theoretical: 80 kDa. Actual: 75 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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