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Anti-Transglutaminase 2 Polyclonal Antibody 2 是一种 Rabbit 抗体,靶向 Transglutaminase 2。Anti-Transglutaminase 2 Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Transglutaminase 2 Polyclonal Antibody 2 是一种 Rabbit 抗体,靶向 Transglutaminase 2。Anti-Transglutaminase 2 Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,160 | 5日内发货 | |
| 100 μL | ¥ 1,970 | 5日内发货 | |
| 200 μL | ¥ 2,790 | 5日内发货 |
| 产品描述 | Anti-Transglutaminase 2 Polyclonal Antibody 2 is a Rabbit antibody targeting Transglutaminase 2. Anti-Transglutaminase 2 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 反应种属 | Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TGM2) Polyclonal Antibody, Unconjugated (TMAB-13732) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human Pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TGM2) Polyclonal Antibody, Unconjugated (TMAB-13732) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Sample: Uterus (Mouse) Lysate at 40 μg Primary: Anti-Transglutaminase 2 (TMAB-13732) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 77 kD Observed band size: 74 kD |
| 应用 | WBIHC-PIHC-FrIF |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 浓度 | 1mg/ml |
| 研究背景 | Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. |
| 免疫原 | KLH conjugated synthetic peptide: human Transglutaminase 2 |
| 抗原种属 | Human |
| 基因名称 | TGM2 |
| 基因ID | |
| 蛋白名称 | Protein-glutamine gamma-glutamyltransferase 2 |
| Uniprot ID | |
| 功能 | Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. |
| 分子量 | Theoretical: 77 kDa. |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |