Anti-Transferrin Receptor/TFRC Antibody (5J722) is a Rabbit antibody targeting Transferrin Receptor/TFRC. Anti-Transferrin Receptor/TFRC Antibody (5J722) can be used in IHC-P,IP.

Monoclonal Rabbit IgG

5J722

Human

1. Immunochemical staining of human TFRC in human placenta with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to trophoblast.

2. Immunochemical staining of human TFRC in human colon carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to intestinal gland.

3. TFRC was immunoprecipitated using:

-Lane A:0.5 mg Jurkat Whole Cell Lysate.

-Lane B:0.5 mg MCF-7 Whole Cell Lysate.

-Lane C:0.5 mg HepG2 Whole Cell Lysate.

-Lane D:0.5 mg Hela Whole Cell Lysate.

-2 µL anti-TFRC rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-TFRC rabbit monoclonal antibody, at 1:100 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 84 kDa.

-Observed band size: 100 kDa

IHC-P,IP

IHC-P: 1:100-1:500; IP: 0.5-2 μL/mg of lysate

Monoclonal

Unconjugated

Recombinant Human Transferrin Receptor / TFRC / CD71 protein (TMPY-01873)

Human