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Anti-Transferrin Receptor/TFRC Antibody (3E660)

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Anti-Transferrin Receptor/TFRC Antibody (3E660)
产品编号 TMAY-01476
别名 transferrin receptor

Anti-Transferrin Receptor/TFRC Antibody (3E660) 是一种 Rabbit 抗体，靶向 Transferrin Receptor/TFRC。Anti-Transferrin Receptor/TFRC Antibody (3E660) 可用于 WB,ELISA,IP。

Anti-Transferrin Receptor/TFRC Antibody (3E660)
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Anti-Transferrin Receptor/TFRC Antibody (3E660)

Rating icon 还可以
Anti-Transferrin Receptor/TFRC Antibody (3E660)
产品编号 TMAY-01476 别名 transferrin receptor

Anti-Transferrin Receptor/TFRC Antibody (3E660) 是一种 Rabbit 抗体，靶向 Transferrin Receptor/TFRC。Anti-Transferrin Receptor/TFRC Antibody (3E660) 可用于 WB,ELISA,IP。

规格价格库存数量
20 μL
¥ 780
5日内发货
100 μL
¥ 2,279
5日内发货
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产品介绍

生物活性
产品描述
Anti-Transferrin Receptor/TFRC Antibody (3E660) is a Rabbit antibody targeting Transferrin Receptor/TFRC. Anti-Transferrin Receptor/TFRC Antibody (3E660) can be used in WB,ELISA,IP.
别名transferrin receptor
Ig Type
Rabbit IgG
克隆号
3E660
交叉反应
Human
验证活性
1. Anti-TFRC rabbit monoclonal antibody at 1:500 dilution.
-Lane A: Hela Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:84 kDa.
-Observed band size:95 kDa.
2. TFRC was immunoprecipitated using:
-Lane A:0.5 mg Jurkat Whole Cell Lysate.
-Lane B:0.5 mg MCF-7 Whole Cell Lysate.
-Lane C:0.5 mg HepG2 Whole Cell Lysate.
-Lane D:0.5 mg Hela Whole Cell Lysate.
-2 µL anti-TFRC rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-TFRC rabbit monoclonal antibody, at 1:200 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 84 kDa.
-Observed band size: 100 kDa.
3. Anti-TFRC rabbit monoclonal antibody at 1:500 dilution.
-Lane A: TFRC konckout Hela Whole Cell Lysate.
-Lane B: Hela Whole Cell lysate.
-Lysates/proteins at 20 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
-Developed using the ECL technique.
-Performed under reducing conditions.
-Predicted band size:84 kDa.
-Observed band size:85 kDa(Validation Experiment)
应用
WB,ELISA,IP
推荐剂量
WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 0.5-2 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human Transferrin Receptor / TFRC / CD71 (rh Transferrin Receptor / TFRC / CD71; TMPY-01873; AAA61153.1; Cys89-Phe 760).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景Transferrin receptor protein 1, also known as transferrin receptor, Trfr, p9, CD71 and TFRC, is a single-pass type II membrane protein that belongs to the peptidase M28 family and M28B subfamily. TFRC / CD71 is a membrane-bound protein expressed in larger amounts in proliferating. The specific expression of TFRC can represent a diagnostic tool or a therapeutic target in solid tumours expressing this antigen. Transferrin receptor is necessary for development of erythrocytes and the nervous system. TFRC / CD71 is regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation. TFRC / CD71 represents a marker of malignant transformation in the pancreas that could be applied as potential diagnostic and therapeutic target.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human Transferrin Receptor / TFRC / CD71 protein (TMPY-01873)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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