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Anti-Transferrin Receptor/TFRC Antibody (3E660)
还可以产品编号 TMAY-01476
Anti-Transferrin Receptor/TFRC Antibody (3E660) 是一种 Rabbit 抗体，靶向 Transferrin Receptor/TFRC。Anti-Transferrin Receptor/TFRC Antibody (3E660) 可用于 WB,ELISA,IP。
Anti-Transferrin Receptor/TFRC Antibody (3E660)
Anti-Transferrin Receptor/TFRC Antibody (3E660)
还可以产品编号 TMAY-01476
Anti-Transferrin Receptor/TFRC Antibody (3E660) 是一种 Rabbit 抗体，靶向 Transferrin Receptor/TFRC。Anti-Transferrin Receptor/TFRC Antibody (3E660) 可用于 WB,ELISA,IP。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Transferrin Receptor/TFRC Antibody (3E660) is a Rabbit antibody targeting Transferrin Receptor/TFRC. Anti-Transferrin Receptor/TFRC Antibody (3E660) can be used in WB,ELISA,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 3E660 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-TFRC rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:84 kDa. -Observed band size:95 kDa. 2. TFRC was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -Lane B:0.5 mg MCF-7 Whole Cell Lysate. -Lane C:0.5 mg HepG2 Whole Cell Lysate. -Lane D:0.5 mg Hela Whole Cell Lysate. -2 µL anti-TFRC rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-TFRC rabbit monoclonal antibody, at 1:200 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 84 kDa. -Observed band size: 100 kDa. 3. Anti-TFRC rabbit monoclonal antibody at 1:500 dilution. -Lane A: TFRC konckout Hela Whole Cell Lysate. -Lane B: Hela Whole Cell lysate. -Lysates/proteins at 20 μg per lane. -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:84 kDa. -Observed band size:85 kDa(Validation Experiment) |
| 应用 | WB,ELISA,IP |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 0.5-2 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human Transferrin Receptor / TFRC / CD71 (rh Transferrin Receptor / TFRC / CD71; TMPY-01873; AAA61153.1; Cys89-Phe 760). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Transferrin receptor protein 1, also known as transferrin receptor, Trfr, p9, CD71 and TFRC, is a single-pass type II membrane protein that belongs to the peptidase M28 family and M28B subfamily. TFRC / CD71 is a membrane-bound protein expressed in larger amounts in proliferating. The specific expression of TFRC can represent a diagnostic tool or a therapeutic target in solid tumours expressing this antigen. Transferrin receptor is necessary for development of erythrocytes and the nervous system. TFRC / CD71 is regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation. TFRC / CD71 represents a marker of malignant transformation in the pancreas that could be applied as potential diagnostic and therapeutic target. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human Transferrin Receptor / TFRC / CD71 protein (TMPY-01873) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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