Anti-TRAIL/TNFSF10 Polyclonal Antibody is a Rabbit antibody targeting TRAIL/TNFSF10. Anti-TRAIL/TNFSF10 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat

1. Sample:
Lung (Mouse) Lysate at 30 μg
U251 Cell Lysate at 30 μg
Primary: rabbit Anti-TRAIL (TMAB-01886) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1:5000 dilution;
Predicted band size: 31 kDa
Observed band size: 26 kDa
2. Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TRAIL Polyclonal Antibody, Unconjugated (TMAB-01886) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TRAIL Polyclonal Antibody, Unconjugated (TMAB-01886) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human TRAIL

Human

TRAIL,TRAIL,Apoptosis,Death Ligands