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Anti-TPH Antibody (6K304) 是一种 Rabbit 抗体,靶向 TPH。Anti-TPH Antibody (6K304) 可用于 WB, IHC-P, IHC-Fr, IP, IF。
Anti-TPH Antibody (6K304) 是一种 Rabbit 抗体,靶向 TPH。Anti-TPH Antibody (6K304) 可用于 WB, IHC-P, IHC-Fr, IP, IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 25 μL | ¥ 790 | 5日内发货 | |
| 50 μL | ¥ 1,380 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
| 产品描述 | Anti-TPH Antibody (6K304) is a Rabbit antibody targeting TPH. Anti-TPH Antibody (6K304) can be used in WB, IHC-P, IHC-Fr, IP, IF. |
| Ig Type | IgG |
| 克隆号 | 6K304 |
| 反应种属 | Human,Mouse,Rat |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TPH Monoclonal Antibody, Unconjugated (TMAB-13693) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TPH Monoclonal Antibody, Unconjugated (TMAB-13693) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TPH Monoclonal Antibody, Unconjugated (TMAB-13693) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. 25 ug total protein per lane of various lysates (see on figure) probed with TPH monoclonal antibody, unconjugated (TMAB-13693) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TPH Monoclonal Antibody, Unconjugated (TMAB-13693) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, Goat Anti-Rabbit IgG H&L-BF594), DAPI (blue) was used to stain the cell nuclei. 6. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TPH Monoclonal Antibody, Unconjugated (TMAB-13693) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, Goat Anti-Rabbit IgG H&L-BF594), DAPI (blue) was used to stain the cell nuclei. |
| 应用 | WBIHC-PIHC-FrIPIF |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IP: 1:20-50; IF: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 组织特异性 | Isoform 2 seems to be less widely expressed than isoform 1. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 研究背景 | Oxidizes L-tryptophan to 5-hydroxy-l-tryptophan in the rate-determining step of serotonin biosynthesis. |
| 免疫原 | A synthesized peptide: human TPH1 |
| 抗原种属 | Human |
| 基因名称 | TPH1 |
| 基因ID | |
| 蛋白名称 | Tryptophan 5-hydroxylase 1 |
| Uniprot ID |
| 分子量 | Theoretical: 51 kDa. Actual: 55 kDa. |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |