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Anti-TOMM20 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 TOMM20。Anti-TOMM20 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,ELISA。
Anti-TOMM20 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 TOMM20。Anti-TOMM20 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,ELISA。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,165 | 5日内发货 | |
100 μL | ¥ 1,970 | 5日内发货 |
产品描述 | Anti-TOMM20 Polyclonal Antibody is a Rabbit antibody targeting TOMM20. Anti-TOMM20 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA. |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | Sample: Lane 1: Mouse Kidney Lysates Lane 2: Mouse Brain Lysates Lane 3: Mouse Heart Lysates Lane 4: Mouse NIH/3T3 Lysates Lane 5: Rat Kidney Lysates Lane 6: Rat Brain Lysates Lane 7: Rat Heart Lysates Lane 8: Human Hela cell Lysates Lane 9: Human THP-1 cell Lysates Lane 10: Human HL-60 cell Lysates Lane 11: Human 293T cell Lysates Primary: Anti-TOMM20 (TMAB-01878) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Mitochondrion outer membrane. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | The mitochondrial preprotein translocases of the outer membrane (Tom) is a multisubunit protein complex that facilitates the import of nucleus-encoded precursor proteins across the mitochondrial outer membrane (1). The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria (2). The import receptors include Tom20 and Tom22, which form a heteromeric receptor complex that initiates the insertion of newly synthesized proteins into the outer membrane and then directs the precursor protein into the GIP (3,4). In yeast, Tom22 is the essential component of the import receptor complex as it functions as both a receptor for the preproteins and serves as a docking point for both Tom20 and the GIP (5,6). Tom22 directly associates with Tom40, the major component of the GIP, and thereby forms a stable interaction between the two core complexes to facilitate the fluid movement of preproteins into the mitochondria (6,7). The insertion of Tom40 into the Tom machinery requires the initial binding of Tom40 to Tom20 and leads to the efficient incorporation of Tom40 precursors into preexisting Tom complexes (2,8) |
免疫原 | KLH conjugated synthetic peptide: human TOM20 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Mitochondrial Biogenesis,Mitochondrial markers,Mitochondrial,Regulation |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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