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Anti-TIMP-2 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 TIMP-2。Anti-TIMP-2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-TIMP-2 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 TIMP-2。Anti-TIMP-2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,170 | 5日内发货 | |
100 μL | ¥ 1,960 | 5日内发货 | |
200 μL | ¥ 2,790 | 5日内发货 |
产品描述 | Anti-TIMP-2 Polyclonal Antibody is a Rabbit antibody targeting TIMP-2. Anti-TIMP-2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
别名 | TIMP metallopeptidase inhibitor 2, DDC8, CSC-21K |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Sheep) |
验证活性 | 1. Tissue/cell: Human endometrium tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-TIMP-2 Polyclonal Antibody, Unconjugated (TMAB-01846) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-TIMP-2 Polyclonal Antibody, Unconjugated (TMAB-01846) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TIMP-2) Polyclonal Antibody, Unconjugated (TMAB-01846) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 4. Sample: Lane 1: Testis (Mouse) Lysate at 40 μg Lane 2: Cerebrum (Mouse) Lysate at 40 μg Lane 3: Testis (Rat) Lysate at 40 μg Lane 4: Cerebrum (Rat) Lysate at 40 μg Primary: Anti-TIMP2 (TMAB-01846) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Secreted. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008]. |
免疫原 | KLH conjugated synthetic peptide: human TIMP-2 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | TIMPs,TIMPs,TIMP,Endothelial mediators/regulators,TIMPs,MMP Inhibitors |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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