Anti-TIMP-1 Polyclonal Antibody 2 is a Rabbit antibody targeting TIMP-1. Anti-TIMP-1 Polyclonal Antibody 2 can be used in WB,IHC-P.

IgG

Human

1. Sample:
Lane 1: TIMP-1 fusion protein 0.2 μg
Primary: Anti-TIMP-1 (TMAB-01845) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 25 kDa
2. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TIMP-1 (CT) Polyclonal Antibody, Unconjugated (TMAB-01845) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Paraformaldehyde-fixed, paraffin embedded (human esophagus cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TIMP-1) Polyclonal Antibody, Unconjugated (TMAB-01845) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human esophagus cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TIMP-1) Polyclonal Antibody, Unconjugated (TMAB-01845) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TIMP-1) Polyclonal Antibody, Unconjugated (TMAB-01845) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human TIMP-1

Human

TIMPs,TIMPs,TIMP,TIMPs,TIMP1 / TIMP2