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Anti-TIMP-1 Polyclonal Antibody

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Anti-TIMP-1 Polyclonal Antibody
产品编号 TMAB-01844
别名 TIMP metallopeptidase inhibitor 1

Anti-TIMP-1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 TIMP-1。Anti-TIMP-1 Polyclonal Antibody 可用于 WB,IHC-P。

Anti-TIMP-1 Polyclonal Antibody

Anti-TIMP-1 Polyclonal Antibody

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Anti-TIMP-1 Polyclonal Antibody
产品编号 TMAB-01844 别名 TIMP metallopeptidase inhibitor 1

Anti-TIMP-1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 TIMP-1。Anti-TIMP-1 Polyclonal Antibody 可用于 WB,IHC-P。

规格价格库存数量
50 μL
¥ 1,175
5日内发货
100 μL
¥ 1,975
5日内发货
200 μL
¥ 2,785
5日内发货
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实验操作小课堂
常见问题解答
化合物带有盐酸盐离子、硫酸盐离子等是否和其本身有什么区别?盐形式和游离态有什么区别?
盐和非盐形式化合物的活性分子是一样的,在生物实验中起到的效果也一致,活性和使用方法都是一样的。只是由于呈盐不同,物理性质比如溶解度会有差异。建议您根据溶解、实验需求进行选择。
如何选择某个靶点的特异性或总的抑制剂?特异性和非特异性的区别是什么?
抑制剂按照特异性分为广谱 pan 和特异性 selective 两种。Pan 为某个靶点总的抑制剂,对所有亚型或整个家族的成员都有抑制作用。Selective 抑制剂针对某个蛋白激酶的某个亚型或家族中某个成员抑制率特别高或有特异性抑制作用。 一般评价一个抑制剂的抑制效率主要看 IC50 值,IC50 值越低,说明抑制剂效率越高。建议您根据以上几个特征进行综合选择,也可联系技术帮您推荐相关抑制剂。
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产品介绍

生物活性
产品描述
Anti-TIMP-1 Polyclonal Antibody is a Rabbit antibody targeting TIMP-1. Anti-TIMP-1 Polyclonal Antibody can be used in WB,IHC-P.
别名TIMP metallopeptidase inhibitor 1
Ig Type
IgG
交叉反应
Human
验证活性
1. Sample:
Lane 1: TIMP-1 fusion protein 0.2 μg
Primary: Anti-TIMP-1 (TMAB-01844) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 25 kDa
2. Tissue/cell: rabbit liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TIMP-1 Polyclonal Antibody, Unconjugated (TMAB-01844) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TIMP-1 Polyclonal Antibody, Unconjugated (TMAB-01844) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TIMP-1 Polyclonal Antibody, Unconjugated (TMAB-01844) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
5. Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TIMP-1 Polyclonal Antibody, Unconjugated (TMAB-01844) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Secreted.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq].
抗原信息
免疫原
KLH conjugated synthetic peptide: human TIMP-1
抗原种属
Human
基因ID
Uniprot ID
研究领域
TIMPs,TIMPs,TIMP,TIMPs,TIMP1 / TIMP2
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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