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Anti-TIE1 Polyclonal Antibody

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货号 TMAB-01838

别名 tyrosine kinase with immunoglobulin-like and EGF-like domains 1, TIE, JTK14

Anti-TIE1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 TIE1。Anti-TIE1 Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P,WB。

Anti-TIE1 Polyclonal Antibody

Anti-TIE1 Polyclonal Antibody

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货号 TMAB-01838 别名 tyrosine kinase with immunoglobulin-like and EGF-like domains 1, TIE, JTK14

Anti-TIE1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 TIE1。Anti-TIE1 Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P,WB。

规格价格库存数量
50 μL
¥ 1,175
5日内发货
100 μL
¥ 1,965
5日内发货
200 μL
¥ 2,780
5日内发货
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产品介绍

生物活性
产品描述
Anti-TIE1 Polyclonal Antibody is a Rabbit antibody targeting TIE1. Anti-TIE1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
别名tyrosine kinase with immunoglobulin-like and EGF-like domains 1, TIE, JTK14
Ig Type
IgG
反应种属
Human (predicted:Mouse,Rat)
验证活性
1. Sample:
A549 (Human) Cell Lysate at 30 μg
HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-TIE1 (TMAB-01838) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 123 kDa
Observed band size: 123 kDa
2. Tissue/cell: human brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TIE1 Polyclonal Antibody, Unconjugated (TMAB-01838) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
verifiedActivityverifiedActivity
应用IFIHC-FrIHC-PWB
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cell membrane; Single-pass type I membrane protein.
组织特异性Specifically expressed in developing vascular endothelial cells.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1 mg/mL
研究背景TIE1/TIE (tyrosine kinase with Ig and EGF homology domains 1) and TIE2/Tek define a new class of the receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin like domains flanking three epidermal growth factor (EFG) like domains followed by three fibronectin type III like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. Human TIE1 cDNA encodes a 1138 amino acid residue precursor protein with a putative signal peptide, an extracellular domain, and a cytoplasmic domain. Human TIE1/Fc, a disulfide linked homodimeric protein, has a calculated molecular mass of approximately 107 kDa. Due to glycosylation, the protein migrates to approximately 160 kDa in SDS PAGE under reducing conditions. TIE1 and TIE2, expressed primarily on endothelial and hematopoietic progenitor cells, play important roles in angiogenesis, vasculogenesis, and hematopoiesis. In developing vascular endothelial cells, TIE1 and TIE2 are specifically expressed. Two ligands that bind TIE have been identified, angiopoietin 1 and angiopoietin 2. Based on gene targeting studies, the in vivo functions of TIE1 are related to endothelial cell differentiation. The receptor tyrosine kinase TIE also plays a role in the survival and integrity of the endothelium.
抗原信息
免疫原
KLH conjugated synthetic peptide: human Tie1
抗原种属
Human
基因名称
TIE1
基因ID
7075
蛋白名称
Tyrosine-protein kinase receptor Tie-1
Uniprot ID
P35590
研究领域
Growth factor receptors,Endothelium,Mesoderm,Receptor Tyrosine Kinases,Endothelial Markers,Mesoderm
功能
Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
化学信息
分子量Theoretical: 123 kDa.
储存&溶解度
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

  • 稀释 计算器
  • 配液 计算器

关键词

JTK-14JTK 14
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