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顶部生物活性储存和溶解信息计算器

Anti-Thioredoxin/TRX Antibody (1K412)

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Anti-Thioredoxin/TRX Antibody (1K412)
产品编号 TMAY-00733
别名 TXN, TRX1, TRX, TRDX, thioredoxin, RP11-427L11.1, MGC61975, DKFZp686B1993

Anti-Thioredoxin/TRX Antibody (1K412) 是一种 Rabbit 抗体，靶向 Thioredoxin/TRX。Anti-Thioredoxin/TRX Antibody (1K412) 可用于 WB,IP。

Anti-Thioredoxin/TRX Antibody (1K412)
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Anti-Thioredoxin/TRX Antibody (1K412)

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Anti-Thioredoxin/TRX Antibody (1K412)
产品编号 TMAY-00733 别名 TXN, TRX1, TRX, TRDX, thioredoxin, RP11-427L11.1, MGC61975, DKFZp686B1993

Anti-Thioredoxin/TRX Antibody (1K412) 是一种 Rabbit 抗体，靶向 Thioredoxin/TRX。Anti-Thioredoxin/TRX Antibody (1K412) 可用于 WB,IP。

规格价格库存数量
100 μL
¥ 2,284
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产品介绍

生物活性
产品描述
Anti-Thioredoxin/TRX Antibody (1K412) is a Rabbit antibody targeting Thioredoxin/TRX. Anti-Thioredoxin/TRX Antibody (1K412) can be used in WB,IP.
别名TXN, TRX1, TRX, TRDX, thioredoxin, RP11-427L11.1, MGC61975, DKFZp686B1993
Ig Type
Rabbit IgG
克隆号
1K412
交叉反应
Human
验证活性
1. Anti-TXN rabbit monoclonal antibody at 1:2000 dilution.
-Lane A: Hela Whole Cell Lysate.
-Lane B: THP-1 Whole Cell Lysate.
-Lane C: A431 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:12 kDa.
-Observed band size:14 kDa.
2. TXN was immunoprecipitated using:
-Lane A:0.5 mg Hela Whole Cell Lysate.
-Lane B:0.5 mg 293T Whole Cell Lysate.
-2 µL anti-TXN rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-TXN rabbit monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 13 kDa.
-Observed band size: 13 kDa
应用
WB,IP
推荐剂量
WB: 1:2000-1:10000; IP: 4-6 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human Thioredoxin / TXN (rh Thioredoxin / TXN; TMPY-02250; P10599; Met1-Val105).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景Thioredoxin, also known as ATL-derived factor, Surface-associated sulphydryl protein, SASP and TXN, is a nucleus, cytoplasm and secreted protein that belongs to the thioredoxin family. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals. Thioredoxin / TXN participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin / TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Thioredoxin / TXN nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Thioredoxin / TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human Thioredoxin / TXN protein (TMPY-02250)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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关键词

TRX-1TRX 1MGC-61975MGC 61975DKFZp686B-1993DKFZp686B 1993

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