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Anti-Thioredoxin/TRX Antibody (1K412)
还可以产品编号 TMAY-00733
Anti-Thioredoxin/TRX Antibody (1K412) 是一种 Rabbit 抗体，靶向 Thioredoxin/TRX。Anti-Thioredoxin/TRX Antibody (1K412) 可用于 WB,IP。
Anti-Thioredoxin/TRX Antibody (1K412)
Anti-Thioredoxin/TRX Antibody (1K412)
还可以产品编号 TMAY-00733
Anti-Thioredoxin/TRX Antibody (1K412) 是一种 Rabbit 抗体，靶向 Thioredoxin/TRX。Anti-Thioredoxin/TRX Antibody (1K412) 可用于 WB,IP。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Thioredoxin/TRX Antibody (1K412) is a Rabbit antibody targeting Thioredoxin/TRX. Anti-Thioredoxin/TRX Antibody (1K412) can be used in WB,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 1K412 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-TXN rabbit monoclonal antibody at 1:2000 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: THP-1 Whole Cell Lysate. -Lane C: A431 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:12 kDa. -Observed band size:14 kDa. 2. TXN was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg 293T Whole Cell Lysate. -2 µL anti-TXN rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-TXN rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 13 kDa. -Observed band size: 13 kDa |
| 应用 | WB,IP |
| 推荐剂量 | WB: 1:2000-1:10000; IP: 4-6 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human Thioredoxin / TXN (rh Thioredoxin / TXN; TMPY-02250; P10599; Met1-Val105). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Thioredoxin, also known as ATL-derived factor, Surface-associated sulphydryl protein, SASP and TXN, is a nucleus, cytoplasm and secreted protein that belongs to the thioredoxin family. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals. Thioredoxin / TXN participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin / TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Thioredoxin / TXN nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Thioredoxin / TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human Thioredoxin / TXN protein (TMPY-02250) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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