Anti-TGF-beta 3 Polyclonal Antibody is a Rabbit antibody targeting TGF-beta 3. Anti-TGF-beta 3 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat)

1. Sample: Ovary (Mouse) Lysate at 40 μg
Primary: Anti-TGF-beta 3 (TMAB-13499) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30'47 kD
Observed band size: 47 kD
2. Blank control (black line): sH-SY5Y.
Primary Antibody (green line): Rabbit Anti-TGF-beta 3 antibody (TMAB-13499)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Negative control (white blue line): PBS
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human TGF-beta 3

Human

TGFB3

Transforming growth factor beta-3 proprotein

Involved in embryogenesis and cell differentiation.